Serial Dilution Lab Report






































5 Serial dilution 7. 50 % aqueous HNO 3 was used as blank solution during the analysis. Yager, Susan M. Into mixing tube C, pipette 5 ml of standard dilution B. You've diluted 100 ul of plasma with 300 ul of distilled water for a 1:4 dilution. Experiment 9 pH of Solutions OUTCOMES After completing this experiment, the student should be able to: calibrate a pH probe for an acidic or basic solution. The laboratory procedure involves making serial dilutions of the sample (1:10, 1:100, 1:1000, etc. (1)donny liang khai chiun b11a056 (2)khor yee heng b11a135 (3)looi kah shien b11a165 (4)yip hon lean b11a438 (5) yong woon sing b11a442 (6) lau su hui b11a565 group sbp 1 date of experiment 8th may 2012 title of experiment experiment 7: staining techniques in microbiology experiment 8. First, the absorption spectrum obtained from methylene blue should peak at an intermediate range approximately equal to 668 nm ("Optical Absorption of Methylene Blue"). Prelab: Connect two PIC18F4321’s are interfaced in the SPI mode. The The findings and conclusions in this report are those of the authors and do not necessarily represent the official. (Pepe 2007) 1. With the HCl solution in the flask with the phenolphthalein, the solution would be colorless initially. Each dilution will reduce the concentration of bacteria by a specific. Dilutions If a 1/8 dilution of the stock solution is made followed by a 1/6 dilution what is the final dilution. Here is an example of a lab report that was actually submitted and received a perfect grade along with step-by-step instructions on writing an effective lab report. It contains an introduction, body and conclusion. It's the only solid state guitar amp I ever really liked. Preparation of the chromium (III) nitrate stock solution i. 5) However Sol. This laboratory activity uses the most widely used medium for common bacterial culture. Lab #1: Pippeting, Buffers, Serial Dilutions and Spectrophotometry Background. Serial dilution plating involves assaying the number of cells in the liquid medium that can form viable colonies on solid culture, a measurement known as the culture's "colony-forming units". Lab practicals schedule Lab report template 5 Lab report 1 Lab report two BIOC1010 Lab report 3 Lab Report 4 BIOC1010. However, if the sample is serially diluted and then plated out on an agar surface in such a manner that single isolated bacteria form visible isolated colonies, the number of colonies can be used as a measure of the number of viable (living) cells in that known dilution. Introduction 3. Instrumentation. Repeat until all dilutions are mixed. The titer is determined by serial dilution, with the reported titer being the last dilution for which the IIF would be identified as positive. Please read them. 1/3 dilutions differ by only 1. Intuitive graphical design. (B) 10-24 Nova Video: What's Living In You? Practice Problems WS: Tues. The visible light absorbance of each of the prepared calibration solutions will be measured. Understand and perform indirect measurement of bacterial growth through spectrophotometer readings and optical density measurements. There are many ways of expressing concentrations and dilution. Since you take the same quantity of reagent from one well to the other, and the first well has the same volume as you put in: you. Basically, a loopful of your original culture is transferred to a tube of liquifeid agar and mixed. 2: Single Isolated Colonies Obtained During the Plate Count. 5 mg/L PO 4-3. serial dilution: [ di-loo´shun ] 1. This method is often used with fungi that form long filamentous colonies that aren’t easily broken up by dilution. Be accessible to the laboratory to provide onsite, telephone, or electronic consultation as needed. Course Snapshot: 680:390 General Microbiology (Lab) -. Examine the effects of serial dilution. When a sample diluted 1/100 is added to a sample diluted 1/10, the final dilution would be: (1/100) x (1/10) = 1/1000. (a) Write the chemical equation which describes the reaction between crystal violet and sodium hydroxide. by charles de farias. Introduction Examples of Antimicrobials Important in Human Medicine Being Used for Animal Treatment, Metaphylaxis or Growth Promotion Figure 2. 75 g/L and Control. Use an appropriate dilution of the mixture with regard to the number of cells to be counted. The visible light absorbance of each of the prepared calibration solutions will be measured. Consider the example of making the first standard at 1/3 the concentration of the known, the next calibrant would be 1/9th the concentration of the known. Dilutions: Principles and Applications Dilutions: Principles and Applications Because solutions in science are often much more concentrated than are desired or can be managed for a given protocol, it is frequently necessary to dilute these solutions to a desired level. In medicine, these skills are necessary to ensuring proper medicine dosage and treatment. Standard obligations in laboratory experiments such as preciseness in calculating and measuring numerous substances, as well as meticulousness are undoubtedly vital. The final volume is the one you want for your final solution, 500. water and measure the pH. Hand in the report section at end of lab. course overview. Signed Procedure 4. Examine the effects of serial dilution. The progression of calibration standard concentration is always a geometric series. These include quantifying the number of bacteria in a sample using plate counts and the development of standard curves for quantitative colorimetric, radiometric, and enzymatic assays. Mix the tube well. 5 mL of stock solution at each step. • prepare a solution of known concentration. To address this PerkinElmer will present a brand-new analytical and software work flow solution, including full sample prep automation, rapid LC/MS/MS analysis and LIMs integration for faster data management to allow labs to run more samples in a workday, which directly relates to higher revenues. Notes on Titering Antibodies 09/11/2007 12:07 PM it's perfectly valid to report a working stock as 20x, as and the prepare a serial dilution series or working. If you haven’t read the previous Smart Knowledge posts in our microbiology series, you can go back and read them from the below link…. This is the case with the juice of the red cabbage. 2, wherein the following is stated with regards to calculation and reporting of results (see below):. Note: See the Pre-Lab Questions for the calculated values for solutions #4 and 5. Put on gloves, fill 9 ml of tryptone broth to ten culture tubes labeled as 10-1 through 10-10. You can then do serial dilutions (1:10) to reduce this to 100, 10, 1, 0. Lab reports can also be written for college classes, as well as other professional areas including engineering and computer sciences. water to use for dilutions ; graduated cylinder (10 ml will work fine. edu/ Corrections, changes or hints to the lab manual: Do your own work. A record of lab work is an important document which will show the quality of the lab work that you have done. , bacterial culture, a chemical, orange juice, etc. Post-labs are due one week after we perform the lab (you can always turn them in sooner). In the 4 test tubes, the following starch solutions were prepared:. (Pepe, 2007) 1. The results show that at 37°C (normal body temperature) and the LB agar plate (rich medium) gives E. Here is an example of a lab report that was actually submitted and received a perfect grade along with step-by-step instructions on writing an effective lab report. Bacterial Culture & Growth Teacher s Guidebook (Cat. 1: Plate count dilution procedure. Calculations are based on the equation for the ionization of the weak acid in water forming the hydronium ion and the conjugate base of the acid. 15 Serial Dilutions (contd) Example of a serial dilution ; 16 Serial Dilutions (contd) In the serial dilution on the previous slide, 1 ml of stock solution is mixed with 9 ml of diluent, for a 1/10 dilution. Introduction It is difficult to know and imagine the size of microorganisms, since they are too small to. The purpose of this lab was just that - to identify two unknown substances by their calculating their protein concentrations and then comparing them to a table of known values. Lecture content also revealed the primary role of Beer's Law in this scientific approach at quantifying metabolite concentrations. Lab reports can also be written for college classes, as well as other professional areas including engineering and computer sciences. 0 dilution steps. This document is not meant to be a substitute for a formal laboratory report. 1/100 dilutions differ by ten-fold. 5 mL) and deionized water (6. Serial dilution is a method intended to reduce the numbers of colonies to a range between 30 and 300; two (2) of the six (6) plates we produced exhibited counts within the targeted range. 00 mL of a stock solution is removed using a. Part B: Concentration and Dilution Lab. This gives 100 times dilution (dilution =10-2). Amylase is present in our saliva, and begins to act on the starch in our food while still in … Continue reading "Amylase on Starch Lab". Serial Dilution. To isolate the bacteria in discrete colonies from the specimen containing more than 1 bacterium. A spread plating procedure was performed in triplicate for each sample using the three highest dilutions. Quantitative estimation of the number of viable microorganisms in bacteriological samples has been a mainstay of the microbiological laboratory for more than one-hundred years, since Koch first described the technique (). After the incubation of plates, the number of cell can be counted. In homeopathy, serial dilutions (called potentisation) are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain. This results in multiplication by two of input A. Less plaques will mean that too many plates will have to be. 5 Serial dilution 7. Notes on Titering Antibodies 09/11/2007 12:07 PM it's perfectly valid to report a working stock as 20x, as and the prepare a serial dilution series or working. To understand this more, let's see this example. Take 1 ml of the phage culture stock that you want to titer and transfer it to the tube titled 10-1 with a pipette. Complete the data table for test tubes #1, 4, and 5. Bacteria in soil occur singly and in aggregates. Step 4: Preparing the bleach Theory: Commercial bleach is approximately 5. A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. Analyzer’s Initial Report would read: Patient Name: George J. edu Summary. Buffer Solutions A buffer solution is one in which the pH of the solution is "resistant" to small additions of either a strong acid or strong base. It may be tempting to think that Vc is the amount of the concentrated solution you have. Using a dilution factor of 5 , continue until the color of the solution is not longer detectable. , bacterial culture, a chemical, orange juice, etc. To get valid results from your samples it is critical to choose appropriate dilutions. The reaction in. View Lab Report - Lab report. Often the standard which is given to you in the lab is far to strong for the experiment and it needs to be diluted. (1)donny liang khai chiun b11a056 (2)khor yee heng b11a135 (3)looi kah shien b11a165 (4)yip hon lean b11a438 (5) yong woon sing b11a442 (6) lau su hui b11a565 group sbp 1 date of experiment 8th may 2012 title of experiment experiment 7: staining techniques in microbiology experiment 8. You will use a protein, bovine serum albimin (BSA) from a stock solution, prepare dilutions, calculate concentrations and set up a standard curve. 0 M HCl with 9. Materials- 6-50mL beakers Acids and Bases. Pre Lab Definitions. S:\PHS\Lab\WP51\Water\Water Manual rev. 005M… Each solution is reduced to one tenth of the previous solutions concentration. >300) and 10 -4 should have 5 (i. Salt Solutions. We worked through the first example together on the board and the students did the dilution using coloured water. Spartans Will. A Step-by-Step Guide for Lab Report 1: Evolution of Cells--Proteinoids and Microspheres Before you begin your report, study a few journal articles. Experimental Errors. Dissolve the 18g of CuSO4 in 100ml of distilled water. Mix test tubes with gentle agitation and. Buffer Properties. Like any titration, neutralization titrations depend on a chemical reaction between the unknown solution and a standard reagent. Determining molecular weight. Centrifuge the broth containing LAB cultures. Name: _____ Block: __ Date: _____ Serial Dilution Lab Objective: The quantity of bacteria present in a sample is an important part of the microbiology report. 3 ml of saline to tubes 2 through 512. The lab experiment goal performed during this experiment was to find out all the factors that affect the intensity of the color solution and how fun colors are that will aid in developing theological background information on how these factors relate to food coloring products. 415 x 2 = 830 mg/dL. The solution in test tube C is 10 times more dilute that the solution in test tube B, which is 10 times more dilute than the solution in test tube A. Obtain a sample of CuSO4 from the front bench. edu Summary. The uniform nature of the process makes it possible to back-calculate characteristics of the original solution based on procedures performed the diluted. Concept Serial dilution is the stepwise dilution of a substance in solution. Reagent dilutions are needed daily in biochemical or immunological lab work. Remember the lateness policy!! 19 Laboratory Reports Con’t 1. Lab 3 - Solutions and Spectroscopy Purpose To determine the concentration of a copper sulfate One of the most common ways to report concentration is in units of molarity. These tubes will be used for viral serial dilutions. This exercise is intended to familiarize us with using scientific notation (to express big and small number easily) and the process of using serial dilutions to make concentrations of known values. The dilution factor chosen for the series of calibration standards is achievable by using serial dilution. *Grade Titration Lab** Serial Dilution Lab - How Hot is TOO Hot??? Lab write-up in lab notebook: Thurs. Light and temperature settings 3. THE PLATE COUNT (VIABLE COUNT) The number of bacteria in a given sample is usually too great to be counted directly. A standard carbon source is glucose, and nitrogen is often provided by peptones (partially digested proteins ), or inorganic salts. doc - 5 - e. Amandeep and Meghan Procedure Step 6 & 7: 6)Your task now is to make a 20 ml, 0. Always use this quantity of sperm as the starting point for dilutions. Hypothesis Predict how the serial dilution of your drink solution will affect the colour, flavor, and other properties of the solution. laboratory report biochemistry and microbiology (bft 1014) members and matric no. Lab Manual. Sewage was dumped in a river and it took five days for it to reach the ocean, hence the five-day incubation requirement in the BOD method. If the stock solution is 10. A title is important to any lab report and it must be clear and specific. Note that the “raw” data (the actual weights recorded at the balance) are. Report: Your lab report should be of the standard format: introduction, method, results, and conclusion/analysis. For quality minimum inhibitory concentration (MIC) service with quick turn around times contact Accugen Laboratories, Inc. , bacterial culture, a chemical, orange juice, etc. course overview. Spread Plate Technique: Principle, Procedure and Results July 28, 2017 Nisha Rijal Bacteriology , Microbiology for Beginners 7 Spread plate technique is a method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample. Learning Goals Construct hybrid molecular orbital diagrams and connect this theory with properties of molecules; Perform computational chemistry calculations to form a theoretical basis for an experiment; Prepare solutions via serial dilutions; Explore limitations in measurements made with the spectrophotometer and analytical, including the differences between absorbance measurements and. prepare a serial dilution of the soil sample. please help!. Anytime you see the abbreviation M you should immediately think of it as mol/L. With the HCl solution in the flask with the phenolphthalein, the solution would be colorless initially. Typical media include plate count agar for a general count or MacConkey agar to count Gram-negative bacteria such as E. Step Starting Concentration (ppm) Starting volume (ml) Water added (ml) Total diluted volume (ml) Final concentration. Outline of procedures: to prepare a standard solution of sodium carbonate and carry out a series of titrations to determine the concentration of hydrochloric acid. Sterile Pasteur pipettes. Spread over the entire surface using the sterile glass stirring rod. pH indicators are usually weak acids or bases themselves. Booker Microbiology 2310 Lab Report 3 Serial Dilutions October 15, 2017 Introduction: A microbe is an organism that is microscopic and submicroscopic, which is too tiny to be viewed under a naked eye. 10 M sodium hydroxide. Dilution (and Concentration) Theory Dilution and concentration theory sounds ominous but you use dilutions and concentrations fairly frequently outside of your "scientific life". Learning Goals: Students will be able to A. 2Department of Environmental Health, Columbia University, New York 10032, U. This exercise is intended to familiarize us with using scientific notation (to express big and small number easily) and the process of using serial dilutions to make concentrations of known values. This Concept Map, created with IHMC CmapTools, has information related to: LAB 4 - ENUMERATION OF MICROORGANISMS, plate count (viable count) tools used 1. Quantitative estimation of the number of viable microorganisms in bacteriological samples has been a mainstay of the microbiological laboratory for more than one-hundred years, since Koch first described the technique (). It is never a good idea to use the title from the lab manual. Part of NCSSM CORE collection: This video shows creation of a series of solutions of different pH by serial dilution. Full text of "Calculations Of Analytical Chemistry" See other formats. Bacterial Culture & Growth Teacher s Guidebook (Cat. Carry out a series of dilutions to obtain standard solutions of 0. 1 LAB – Solubility Lab Procedures. Page 3 Note about Vc, and a hidden assumption. Most specimens have high enough numbers of microorganisms that the specimen has to be serially. Slow growing bacteria may not result in visible colonies within a reasonable time frame (1-2 weeks). See Table 1. Make 1:20 dilutions of each of the above solutions. An example of a poorly written method section from a biology report We did a serial dilution by pipetting 0. Cover page 2. 5 x 103 CFU/ml (3,500 CFU/ml) 9 ml 3. Your report should include: A description of the sample and its preparation for the titration (ion-exchange resin, dilution). Upon completion of this lab, the student should be able to • proficiently calculate molarities for solutions. You can see from the table below that these dilutions will allow you to apply treatment up to 20 mg/L in the sample beaker. The primary objective of this experiment is to determine the concentration of an unknown nickel=(II) sulfate solution. On the contrary, serial dilution is a lab technique in which a substance is decreased in concentration in a series of proportional amounts. Data was recorded based on the amount of agglutination in each dish. It is impossible to make an exact measurement. Besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. Each dilution will reduce the concentration of bacteria by a specific. The Science vehicles at Westminster College will transport. Salinity affects soil ecosystems because high levels of salt can create an imbalance of plant nutrients in the soil which will prevent essential organisms such as plants and. To perform three dilutions with your prepared solution. Keep in mind that the reader may not be familiar with your experiment. 23rd One Full Report, One Technical Report due: Nov. Determining the Concentration. DOB 12/24/1949. Your report should include: A description of the sample and its preparation for the titration (ion-exchange resin, dilution). A test for the presence of an antibody was made on each solution. Since the introduction of the first continuously variable micropipette in 1971, more than three million Thermo Scientific Finnpipettes have been sold in 150 countries. It is used to determine the data usability of the samples. In a real lab, this experiment would take a week or more to complete, but in this simulation it only takes 30. Summary & Discussion. The final volume is the one you want for your final solution, 500. If you are going to measure results for four dilutions, it is a waste of time and materials to make two of them almost the same. A dilution is prepared by adding a specific amount of a concentrate to water to obtain a new volume and concentration. In general, more assessable data are obtained with a greater number of dilutions, test organisms, and test replicates. Calculate the concentrations of the diluted solutions in test tubes #2 and 3 using Equation 2 from the Background section. Dilutions of 10-mL standard phosphate solutions were prepared in increasing concentrations of 0. Section 2 Performing this lab, a serial dilution was set up for the red and blue dyes. The first tube will be undiluted serum. Serial dilution is the simplest approach where cells are serially diluted to approximately one cell per microliter (Yuan et al. The pour plate technique, also sometimes called the loop dilution method, involves the successive transfer (serial dilution) of bacteria from the original culture to a series of tubes of liquified agar. Experiment 9 pH of Solutions OUTCOMES After completing this experiment, the student should be able to: calibrate a pH probe for an acidic or basic solution. For part A and D of the lab, the results and calculations were consistent, but part B and C showed little consistency. 3 BCA™ Protein Assay Kit. In homeopathy, serial dilutions (called potentisation) are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain. Materials- 6-50mL beakers Acids and Bases. Learn techniques for serial dilution and cell counting Understand how to set up experimental design and hypothesis Background: After observing dense cultures of tetrahymena last week, to better observe the organisms we must dilute the sample into more easily observable number of organisms. A serial dilution is an evenly and geometrically paced series of doses that starts with a concentrated substance that becomes increasingly diluted at each step down. oo1 M NaOH was made by taking 1 mL of 0. Moreover, this lab involved a serial dilution, which is a method, used to determine an antibody concentration by measuring the change of the color spectrometically (e. Wastewater Laboratory Basics sampling, ammonia, BOD, chlorine residual, fecal coliform, pH, solids IWEA Laboratory Workshop August 8, 2013. To perform the ANA blood test, sometimes called FANA (fluorescent antinuclear antibody test), a blood sample is drawn from the patient and sent to the lab for. 9 ml broth into labelled tubes, then adding 2 drops (0. Microbiology Lab Safety Instructions. When the color changes to the specified color, the titration has reached endpoint. Determination of Fe by Redox Titration Matt Cuff Quant 320L October 21, 2011 Abstract In this experiment the percent of iron in an unknown sample will be determined by using a redox titration and then compared to a different method. Serial dilution is a method intended to reduce the numbers of colonies to a range between 30 and 300; two (2) of the six (6) plates we produced exhibited counts within the targeted range. You are on page 1 of 3. Available to play online. Record the amounts of water and iron stock solution you used in Table 4. Mass of 100-ml volumetric flask, s 2. The exact format required depends upon the type of written communication and often will vary from source to source. Full Lab Report Due: Nov. Following a period of incubation, the wells are examined for growth. Serial Dilutions 1 T’Aariel D. The smallest serial dilution process is three 1. It was positive for the 1/2 and 1/4 dilutions but negative for the 1/8 and 1/16 dilutions. 10X serial dilution of N&P in pond water 3. 75 g/L and Control. This procedure is called a serial dilution. Simple dilutions are practical when the dilution factor is about 100-fold or less. Obviously, the BOD test is not the most scientifically based test we perform. She starts with a 1mg/ml sample and 10 tubes, (labelled 1-10) each containing 2 ml buffer. The diversity of the salmonella microbiology lab report that will help manufacturers of medical device testing, general analytical tests, pharmaceutical testing, processed tissue testing and microbiology laboratory helps us to make certain that the central part has been clean of the salmonella microbiology lab report, which remained sterile. coli culture: Fill 7 tubes with 4. Cu were prepared by serial dilution of the 1000. Serial Dilutions and Pipetting. Serial dilutions are a progressive series of dilutions. Using your calculations from question #4 above, measure out the correct amount of solid Kool-Aid powder (grams) into the assigned cup to make a 0. Dilutions of 10-mL standard phosphate solutions were prepared in increasing concentrations of 0. Because of this, a variety of methods have been developed. Determining the Concentration. Materials- 6-50mL beakers Acids and Bases. A culture of microbes can be diluted in the same fashion. A serial dilution of extracellular extracts (0x, 2x, 10x, 50x and 100x) are prepared with total of 5 ml for each serial dilution. 1/100 dilutions differ by ten-fold. Safety Rules Glassware & Techniques Keeping a Lab Notebook Writing a Lab Report. To estimate the number of bacteria in a gram of soil, the soil must be both diluted and mixed thoroughly so that the aggregates are broken up such that a suspension of single cells is achieved. LAB REPORT OF MICROBIOLOGY. Pre Lab Definitions. If you find this experiment more interesting and at least another student who shares your choice, you should discuss it with the instructor on the last day of your Lab #1 or #2 whichever comes last. Dissolve the 18g of CuSO4 in 100ml of distilled water. The smallest serial dilution process is three 1. (10*10*10 = 1000 fold). Errors Making multiple calibration standards for your lab equipment means measuring out a solution of known concentration and diluting it to make a series of lower concentrations. Also spot several DNA standards of known concentration. (1)donny liang khai chiun b11a056 (2)khor yee heng b11a135 (3)looi kah shien b11a165 (4)yip hon lean b11a438 (5) yong woon sing b11a442 (6) lau su hui b11a565 group sbp 1 date of experiment 8th may 2012 title of experiment experiment 7: staining techniques in microbiology experiment 8. Put on gloves, fill 9 ml of tryptone broth to ten culture tubes labeled as 10-1 through 10-10. The student puts 1ml of sample solution into tube 1. Doubling Dilutions "Doubling dilutions" are very popular. During serial dilution of the soil, soil particles can settle out (fall to the bottom), so the true aliquot of soil is not passed on into the next dilution. After the incubation of plates, the number of cell can be counted. 1/3 dilutions differ by only 1. Serial Dilution Many procedures performed in modern biology and chemistry laboratories require sets of solutions that cover a range of concentration * s. Choose from 184 different sets of serial dilutions flashcards on Quizlet. Spread Plate Technique: Principle, Procedure and Results July 28, 2017 Nisha Rijal Bacteriology , Microbiology for Beginners 7 Spread plate technique is a method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample. Record the exact amount that you used in Data Table B. An Explanation of Sensitivity and the LLD, LLOQ, and ULOQ of a Multiplex ELISA. To prepare solutions through serial dilution, 1. Dilute yeast sample to an appropriate concentration. Making Molar Solutions and Dilutions Objectives Your performance will be satisfactory when you are able to: • Correctly prepare a solution of a given molarity leaving a clean lab area. Carefully weigh the desired amount of CuSO 4 · 5 H 2 O on a weighing paper or in a small beaker as shown in the Using an Analytical Balance video under Instructional Videos and as described in the Analytical Balance section of the Lab Equipment of this lab manual. do your best to devise a serial dilution procedure. Experiment 6: THE RATE LAWS OF AN IODINE CLOCK REACTION 59 Purpose: The rate law for the reaction of an “iodine clock” reaction is to be established. In homeopathy, serial dilutions (called potentisation) are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain. dilutions are made. Calculate the concentration of glucose in each tube using the following equation and record it in your assignment: V1C1 = V2C2. Description: In this exercise, the method of successive dilutions was demonstrated using HCl, NaOH, a pH meter, and universal indicator solution. Hyde and Wellcome W. Amylase is present in our saliva, and begins to act on the starch in our food while still in … Continue reading "Amylase on Starch Lab". Lab Report-yeast Mould. Consider the example of making the first standard at 1/3 the concentration of the known, the next calibrant would be 1/9th the concentration of the known. In the Bradford assay, we used the dye Coomassie G-250 which binds to proteins mostly at arginine but also at tryptophan, tyrosine, histidine and phenylalanine. LAB # 13 Dr. WORKING DILUTION PROBLEMS It is a common practice to determine microbial counts for both liquid and solid specimens---suspensions of E. Serial Dilution Visual A 1:10 dilution of 6. 00005 drops/mL. Introduction to Aseptic techniquesIn microbiological and biochemical engineering studies, one almost always deals with a pure culture or a mixture of known cultures, except perhaps in waste water treatment studies. Experimental Errors. After the incubation of plates, the number of cell can be counted. Depending on whether how low or high the dilutions in microbiology can produce for medical advancements. What is the MIC? The Minimum Inhibitory Concentration (MIC) is determined by inoculating the organism into a series of test wells, usually 4 to 6, that contain a standard amount of broth and serial dilutions of the antibiotic being tested. 55%, so this seems very valid. Hacks assists you in multiple lab tasks: • Learn scientific techniques and practical tips and tricks ,which will help you to improve the output of your experiment. color, texture, size, elevation, etc. Once organism is diluted out by certain dilution factor, it is allowed to inoculate on suitable nutrient media in Petri dished. Spread Plate Technique: Principle, Procedure and Results July 28, 2017 Nisha Rijal Bacteriology , Microbiology for Beginners 7 Spread plate technique is a method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample. In this device, red light from the LED light source will pass through the solution and strike a photocell. 005M… Each solution is reduced to one tenth of the previous solutions concentration. Review the concept that serial dilutions reduce the concentration of the sample in small steps or fractions. Carry out a series of dilutions to obtain standard solutions of 0. Water Chemistry II: Spectrophotmetry & Standard Curves Standard curves are graphs of light absorbance versus solution concentration which can be used to figure out the solute concentration in unknown samples. The lower dilutions are used because actinomycetes are typically present as 1/10 th of the bacterial population (B = 10-2, C = 10-3, D = 10-4). Conduct three trials for each dilution. Davidsonb a RDECOM, Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD 21010, USA b Science and Technology Corporation, Belcamp, MD 21017, USA article info abstract Article history: Received 9 May 2014 Received in revised form 29 August 2014. Pipettes [Manual Cell Counts] Historically, micropipettes are used to make dilutions of whole blood. 25 Sum Up Progress on the Learning Goals. Davidsonb a RDECOM, Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD 21010, USA b Science and Technology Corporation, Belcamp, MD 21017, USA article info abstract Article history: Received 9 May 2014 Received in revised form 29 August 2014. When the color changes to the specified color, the titration has reached endpoint. 25 M NaOH solution (this is read as 0. standards of a common protein such as bovine serum albumin (BSA). The report you got from the instrument is the diluted sample so you must MULTIPLY the result you got by the dilution factor- this time by 2. Procedure: 1. ) Follow the same steps for the 10-5 and 10-4 dilutions. 25 M NaOH solution (this is read as 0. What is concentration? Concentration refers to the amount of solute that is dissolved in a solvent. Please note that we report all of our results as per international standard, ISO 7218, ‘Microbiology of Food and Animal Feeding Stuffs – General Rules for Microbiological Examinations’. edu; office at ESCIE 1. water and measure the pH. If you are going to measure results for four dilutions, it is a waste of time and materials to make two of them almost the same. Box 117 Rockford, IL 61105 23225 23227 1296. While you may already make solutions in the lab by following recipes, we hope this Webinar will help you understand the concepts involved so that you can. In chemistry, concentration is usually measured by the number of moles of substance dissolved in a liter of liquid. Add 5 mL of double-strength MRS with 1 mL of cultures containing spoilage/pathogenic bacteria and vortex the mixture. Preparing a Stock Solution ing a Stock Solution of Known Concentration ametric flask, g 59. For isolation of LAB, serial dilution agar technique was used. 5 x 10 4 3, 1. Because the microorganisms used in this class are potentially harmful (BL II), NO eating or drinking is allowed in the lab. enumeration. Introduction. Learning Goals: Students will be able to A. University. The solution in test tube C is, therefore, 10 x 10 = 100 times more dilute than the solution in test tube A. The Quality Control Section of ESS Laboratory's analytical report is located after the Sample Results. Lab reports can also be written for college classes, as well as other professional areas including engineering and computer sciences. *Grade Titration Lab** Serial Dilution Lab - How Hot is TOO Hot??? Lab write-up in lab notebook: Thurs. Fungal tests are used to help detect and diagnose a fungal infection, to help guide treatment, and/or to monitor the effectiveness of treatment. Hacks provides you with tools, calculators and libraries for your daily work at the lab. Helpful? 130 26. Staff A-Z | Information for new staff | Contact us. Microbiology Lab Safety Instructions. IC50 assays, commonly used to evaluate drug efficacy, and assay development. Remember the lateness policy!! 19 Laboratory Reports Con't 1. Pour contents of "snap pack" into the water sample. Source: "Infuuszakjes" by Harmid is in the public domain. One of the most common series doubles the dilution factor with each transfer (1:2, 1:4, 1:8. A pH indicator is a chemical compound that is added in small amounts to a solution so that the pH (acidity or alkalinity) of the solution can be determined easily. For example, how would you prepare 500. The results are reported in cells/mL. 001 mL is 1/1000th of a mL. 6th Nov 2nd Fluoride in Groundwater and Toothpaste Ion Selective Electrode Technical Report – Data, Calculations, and Results Due: Nov. 0 dilution steps. Ubiquity and Cultural. Dilutions: Explanations and Examples of Common Methods. D Calculate protein concentration in µg/µl of original sample; include mean and standard deviation. Preparation of the chromium (III) nitrate stock solution i. Propagation of Errors in Dilution Problems When a calculation is done in lab, it is important to round the result to the number of significant figures indicated by the precision of the measurements used in the calculation. oo1 M NaOH was made by taking 1 mL of 0. In this lab, a serial dilution will be made of a sample of bacteria, and then those dilutions will be used to culture bacteria in order to estimate their numbers. An example title might be "Finding an Unknown Dilution by Measuring Optical Density of Serial Dilutions. Additional Background & Technique Information to Supplement the Lab Manual Two techniques were employed to gather the data provided: (1) serial dilutions and (2) UV/Vis. Probably the most common application in biology of this technique is in the measurement of the concentration of a compound in solution. Preparing a Stock Solution ing a Stock Solution of Known Concentration ametric flask, g 59. a) Run the lab as a Java Applet in a web browser by clicking on “Run the applet >>”. To prepare solutions through serial dilution, 1. Sewage was dumped in a river and it took five days for it to reach the ocean, hence the five-day incubation requirement in the BOD method. • prepare a dilute solution from a more concentrated one. LAB REPORT OF MICROBIOLOGY. Red cabbage contains a water-soluble pigment called anthocyanin that changes color when it is mixed with an acid or a base. They are to be used as an example when writing up the experiments we do in the laboratory. Lab-Lecture Notes. Microbiology Lab Safety Instructions. Plates were incubated at room temperature (ca. To isolate the bacteria in discrete colonies from the specimen containing more than 1 bacterium. C Serial dilution of unknown to find proper dilution that gives A 595 values between 0. For part A and D of the lab, the results and calculations were consistent, but part B and C showed little consistency. D Calculate protein concentration in µg/µl of original sample; include mean and standard deviation. Serial Dilutions 1 T’Aariel D. • Do parallel and serial dilutions and distinguish between the two. Serial dilutions are usually made in multiples of 10 –– termed a ““tenfold dilution”” –– but can be made in any increment you choose, such as twofold, fivefold, or hundredfold dilutions. 5 mL) and deionized water (6. This process keeps the amount of solute constant, but increases the total amount of solution, thereby decreasing its final concentration. PIPETING & DILUTIONS Dilutions are used many times during the semester in the microbiology lab, for a variety of purposes. On the narrow stem is a calibration mark. 9)Label four test tubes with the four different concentrations you determined in the lab. , adding table salt to water), but the. The instructions below are designed to keep you safe in the laboratory. Intuitive graphical design. Sterile Pasteur pipettes. As illustrated here with a serial dilution of dye, each dilution contains less of the original sample. Introduction Microorganism is an organism that is microscopic or submicroscopic, which is too small to be seen under naked eyes. The relationship between the concentration of the solutions and their absorbance will be investigated. 1/3 dilutions differ by only 1. Serial dilutions and plating of cells Lenzmeier Research Laboratory Page 1 of 2 Serial dilutions of microbial cells and for spot-tests 1. Tips for handling specific types of data: Dilutions. 1 mL of yeast solution will be added to 9. All services are performed at our one-stop facility near Philadelphia, PA (USA). This is done using a set of standards containing varying levels of an analyte in high enough and low enough concentrations so as to span the entire range of the test system. To make a dilution, you simply add a small quantity of a concentrated stock solution to an amount of pure solvent. Spread 100 µl of each dilution onto its corresponding plate. Serial Dilution Lab In this unit we will be discussing measurements of concentration that us e both very big and very small numbers. Macrobroth dilution tests : A serial two-fold dilution of antibiotic are made in test tubes from 0 to maximum concentration that is achieved in vivo without toxic effect on patient. Spartans Will. In the vitamin solution test tube, put 5 mL of the vitamin solution. The diversity of the salmonella microbiology lab report that will help manufacturers of medical device testing, general analytical tests, pharmaceutical testing, processed tissue testing and microbiology laboratory helps us to make certain that the central part has been clean of the salmonella microbiology lab report, which remained sterile. Chem 405 Biochemistry Lab I Experiment 1 Biochemical Calculations, Graphing, pH and Pipetting Calculations: Preparation and handling solutions is an essential part of experimental biochemistry. University College London. As shown in the illustration below, it begins when 1 mL of the bacterial sample is added to 9 mL, and it is mixed together (creating a 10-1 dilution). Lab Session One 1. Minerals and vitamins may also be provided, according to the growth requirements of the bacteria. The sections required vary from laboratory to laboratory but the standard outline for most lab reports in the biological science include: title, your name, purpose of the experiment, methods, results, discussion and conclusion, references. ? Michigan State University. 1013g Final Volume of NaOH stock solution A=50mL Concentration of NaOh stock solution = ? For that part^ , how do I find the cocentration of NaOH stock solution A? PART B Beaker label B Volume of preceding solution taken=35mL Initial Concentration of NaOH solution(M)=?. Solutions, Dilutions, Concentrations and Molarity A serial dilution is a series of simple dilutions The findings and conclusions in this report are. 50 % aqueous HNO 3 as diluent. The titer is determined by serial dilution, with the reported titer being the last dilution for which the IIF would be identified as positive. dilution of the sample is necessary. BOD-04: The sample dilutions set-up for the BOD analysis did not meet the final DO reading with a value of equal or greater than 1 mg/l, due to matrix. The use of a contaminated. Always use this quantity of sperm as the starting point for dilutions. ENUMERATION OF BACTERIA IN A POPULATION: USING SERIAL. Neubauer and coverslip. Dilution factor (D. Calculate the concentration of glucose in each tube using the following equation and record it in your assignment: V1C1 = V2C2. All squares represent 1. ) Follow the same steps for the 10-5 and 10-4 dilutions. Name: _____ Block: __ Date: _____ Serial Dilution Lab Objective: The quantity of bacteria present in a sample is an important part of the microbiology report. If you find this experiment more interesting and at least another student who shares your choice, you should discuss it with the instructor on the last day of your Lab #1 or #2 whichever comes last. Fluorescent Western Blotting Antibodies. by phone at 800-282-7102 or by email at [email protected] DOB 12/24/1949. Ranking process. • 2 plastic dropping pipettes for use in serial dilution procedure—one for water and one for transferring dye solution from one well to another. Show your work. For example, in 1/10 serial dilutions starting with a stock solution of 5M solute concentration, the serial dilution would be comprised of 5M, 0. FIRST PERIOD 1. coli can grow, temperature and difference in medium. Finish Serial Dilution Lab. Protocol for using serial dilutions in the microbiology lab. Skilled phlebotomists are available to draw blood samples from patients both in the hospital setting, and in an outpatient setting at our various patient service centers throughout the region. Sewage was dumped in a river and it took five days for it to reach the ocean, hence the five-day incubation requirement in the BOD method. We generated a standard curve for a set of albumin samples. This method is often used with fungi that form long filamentous colonies that aren’t easily broken up by dilution. The colligative properties really depend on the escaping tendency of solvent molecules from the liquid phase. Solutions, Dilutions, Concentrations and Molarity A serial dilution is a series of simple dilutions The findings and conclusions in this report are. Serial Dilution Simulation Lab - Free download as PDF File (. Single Dilution Exercise. For example, what volume of media would be needed to dilute 100µL of a culture to the 108 - dilution used in this lab in a single step. Chemistry on a Cart. environmentalexpress. Prior to lab review: Serial dilution. – Effectively a 49 x 48 tube serial dilution Using the Thomas formula: (MPN is an approximate, deviates from the MPN table and is not significant) Number of +ve tubes = 96 Volume of –ve tubes = 0. How the Test Is Performed To perform the ANA blood test, sometimes called FANA (fluorescent antinuclear antibody test), a blood sample is drawn from the patient and sent to the lab for testing. Tap one of the "snap packs" to ensure that all of the Colilert ® reagents are in the bottom part of the pack. 9)Label four test tubes with the four different concentrations you determined in the lab. the lab report. Objective- to determine the pH range and the colors associated with several indicators. Serial dilution is a simple yet efficient technique to determine the number of cells or organisms in a concentrated sample. A record of lab work is an important document which will show the quality of the lab work that you have done. The relationship between the concentration of the solutions and their absorbance will be investigated. The objective of the serial dilution method is to estimate the concentration (number of colonies, organisms, bacteria, or viruses) of an unknown sample by counting the number of colonies cultured from serial dilutions of the sample, and then back track the measured counts to the unknown concentration. They detect the presence of hydronium ions (H 3 O +) or hydrogen ions (H +). The comparison of methods experiment is critical for assessing the systematic errors that occur with real patient specimens. To make a dilution, you simply add a small quantity of a concentrated stock solution to an amount of pure solvent. University. This general microbiology practical lecture explains the serial dilution techniques in pour plate method to isolate bacteria. Instead, they make concentrated stock solutions and then make dilutions of those stocks as necessary for a given experiment. A student wants to make serial dilution of a sample. Th e following sections should appear in your lab reports. Booker Microbiology 2310 Lab Report 3 Serial Dilutions October 15, 2017 Introduction: A microbe is an organism that is microscopic and submicroscopic, which is too tiny to be viewed under a naked eye. A report is a structured document that takes information and presents it in an objective and succinct manner. (1)donny liang khai chiun b11a056 (2)khor yee heng b11a135 (3)looi kah shien b11a165 (4)yip hon lean b11a438 (5) yong woon sing b11a442 (6) lau su hui b11a565 group sbp 1 date of experiment 8th may 2012 title of experiment experiment 7: staining techniques in microbiology experiment 8. Lab report 1 Richard Montez MCB 3020L Monday 2 pm June 15, 2013 Abstract: Bacteria is a very diverse creature, and can grow in extreme conditions. Matrix Spike recovery was within the 75-125% control limits except for Sb (MS/MSD), Mg, Pb (MS) and Mn (MSD). Determination of Fe by Redox Titration Matt Cuff Quant 320L October 21, 2011 Abstract In this experiment the percent of iron in an unknown sample will be determined by using a redox titration and then compared to a different method. To quantitate mixtures of DNA fragments or DNA of multiple sizes, spot samples on a 1% agarose slab gel containing 0. txt) or read online for free. A leader in pipetting For over 35 years, our goal has been to help customers in research and clinical laboratories to improve the speed, accuracy and precision of their pipetting. 2: Single Isolated Colonies Obtained During the Plate Count. Amandeep and Meghan Procedure Step 6 & 7: 6)Your task now is to make a 20 ml, 0. Analyzer's Initial Report would read: Patient Name: George J. Salinity affects soil ecosystems because high levels of salt can create an imbalance of plant nutrients in the soil which will prevent essential organisms such as plants and. The animation below hows how to dilute a 3M solution by 1/100 and 1/100. Purpose: The purpose of this lab was to teach students how to use micropipettes, how to make serial dilutions, and how to determine the number of cells in a diluted solution as well as a stock solution. It is critical that you master both serial and simple (single) dilutions. Each group will have 1 strain of LAB and 3 strains of spoilage/ pathogenic organisms. Serial dilutions of samples are made using EMEM10 as the diluent in 8-well Lab-Tek. If you have any questions about safe laboratory practices, ask your instructor. Initial sample 3. 0 mL for the pure culture and 0. Illustration of the dilutions made. 9 mL to obtain more convenient ten-fold dilution steps. Use a sterile pipet to place 2 mL of sterile water into one tube and 1 ml into each of six more tubes. Showing top 8 worksheets in the category - Lab Report. When just enough of the NaOH solution has been added to. Estimation method for serial dilution experiments Avishai Ben-David a,⁎, Charles E. Duration Describe measuring algal growth. Lab Report 12: Simple Harmonic Motion, Mass on a Spring 04/20/12 James Allison section 20362 Group 5 James Allison, Clint Rowe, & William Cochran Objective: For our final lab of associated with physics I, we will dissect the motions of a mass on a spri. Part of NCSSM CORE collection: This video shows creation of a series of solutions of different pH by serial dilution. Red cabbage contains a water-soluble pigment called anthocyanin that changes color when it is mixed with an acid or a base. You will be using the colorimeter shown in Figure 1. Serial dilutions of samples are made using EMEM10 as the diluent in 8-well Lab-Tek. Note your dilution factor. The supernatant will be used as extracellular extracts. if you are interested with the sporulating bacteria,heat shock the solution at 80 degrees for15 minutes to kill the vegetative bacteria. r-October 1, 1977 - r~arch. GENERAL MICROBIOLOGY (BIOL3401) LABORATORY SYLLABUS SPRING 2017. Equation: R2 value: Calculate the MnO4 – concentration in the diluted Unknown Solution using the equation of the trendline and the measured solution absorbance. For example, when you are making orange juice from a frozen concentrate, you mix 1 can of concentrate with 3 cans of water. Calculate the molar mass of sucrose. To load the assignment, select "Load Homework" from the "File" menu, and select "Acids and Bases : Method of Successive Dilutions ". The scope of a report varies depending upon the. In the lab, correct dilutions of solutions are critical to performing accurate science. ∗email:[email protected] Answer to: What is the importance of serial dilution? By signing up, you'll get thousands of step-by-step solutions to your homework questions. Hopfully when going into the lab, you are able to know the following skills. (Laboratory Report 3 covers Experiments 2, 3, and 4) The Final Laboratory Report is a revision of Report 3, and thus also covers Experiments 2, 3, and 4. serial dilutions vs direct dilutions. Serial Dilution - Free download as Word Doc (. The goal is to have at least one sample deplete by more than 2. Record the pH in your lab notebook. For example, what volume of media would be needed to dilute 100µL of a culture to the 108 - dilution used in this lab in a single step. (Lab period 1) Spectrophotometry: Absorption spectra and the use of light absorption to measure concentration Spectrophotometry is a procedure that is frequently utilized in biological laboratories. It is important to know how to design and perform dilutions which are accurate and which meet the needs of the situation. 2) Count five seeds per bag or Petri dish. Ranking process. 13th Group Rotations Next Four Weeks (Two Week Labs) Lab start dates: Nov. 0 mL for the pure culture and 0. Because of this, a variety of methods have been developed. The visible light absorbance of each of the prepared calibration solutions will be measured. 25 M NaOH solution (this is read as 0. Preparing a Stock Solution ing a Stock Solution of Known Concentration ametric flask, g 59. To perform three dilutions with your prepared solution. Label your fi gures and tables. This activity was created as an accompaniment to an in-class demonstration of the method of successive dilutions using HCl, NaOH, a pH meter, and universal indicator solution. Biotechnology Explorer™ pGLO™ Bacterial Transformation Kit Catalog #166-0003EDU explorer. You can use serial dilutions of a solution of known concentration to calibrate lab equipment and ensure its accuracy. Serial dilution is one of the core foundational practices of homeopathy, with "succussion", or shaking, occurring between each dilution. Acid Base Indicators Lab. Molarity and Serial Dilutions Teacher Handout Module Overview One of the most important skills in science is to calculate dilutions. This liquid has a blue-violet color, but when it comes in contact with acidic substances it becomes red, while in contact with basic substances it becomes green and even yellow. You've diluted 100 ul of plasma with 300 ul of distilled water for a 1:4 dilution. If you find this experiment more interesting and at least another student who shares your choice, you should discuss it with the instructor on the last day of your Lab #1 or #2 whichever comes last. Bacterial Enumeration-Dilutions and Plate Counts. Procedure to making Kool-Aid (for every lab group of 3-4 people): 1. – Effectively a 49 x 48 tube serial dilution Using the Thomas formula: (MPN is an approximate, deviates from the MPN table and is not significant) Number of +ve tubes = 96 Volume of –ve tubes = 0. Soon after collection the water sample was preserved by the addition of 50 (v/v) % aqueous HNO 3 until the pH was below 2. ! • Streak out a fresh plate for Tues (need not be quadrant streak) • Unknown Assignment Report, due June 8th (change of date) • Is worth about 33% of your lab grade, more than all the practicums combined! Sct live load software for a brother. This experiment is the subject of a formal lab report. 5 x 103 CFU/ml (3,500 CFU/ml) 9 ml 3. laboratory report biochemistry and microbiology (bft 1014) members and matric no.


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